Heterofucans from Dictyota menstrualis have anticoagulant activity

Heterofucans from Dictyota menstrualis have anticoagulant activity

Autor Albuquerque, Ivan Rui Lopes Google Scholar
Queiroz, Karla Cristiana Souza Google Scholar
Alves, Luciana Guimarães Autor UNIFESP Google Scholar
Santos, Elizeu Antunes dos Autor UNIFESP Google Scholar
Leite, Edda Lisboa Autor UNIFESP Google Scholar
Rocha, Hugo Alexandre Oliveira Autor UNIFESP Google Scholar
Instituição Universidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Biotecnologia de Polímeros Naturais
Universidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Glicobiologia
Universidade Federal de São Paulo (UNIFESP)
Resumo Fucan is a term used to denote a family of sulfated L-fucose-rich polysaccharides which are present in the extracellular matrix of brown seaweed and in the egg jelly coat of sea urchins. Plant fucans have several biological activities, including anticoagulant and antithrombotic, related to the structural and chemical composition of polysaccharides. We have extracted sulfated polysaccharides from the brown seaweed Dictyota menstrualis by proteolytic digestion, followed by separation into 5 fractions by sequential acetone precipitation. Gel electrophoresis using 0.05 M 1,3-diaminopropane-acetate buffer, pH 9.0, stained with 0.1% toluidine blue, showed the presence of sulfated polysaccharides in all fractions. The chemical analyses demonstrated that all fractions are composed mainly of fucose, xylose, galactose, uronic acid, and sulfate. The anticoagulant activity of these heterofucans was determined by activated partial thromboplastin time (APTT) using citrate normal human plasma. Only the fucans F1.0v and F1.5v showed anticoagulant activity. To prolong the coagulation time to double the baseline value in the APTT, the required concentration of fucan F1.0v (20 µg/ml) was only 4.88-fold higher than that of the low molecular weight heparin Clexane® (4.1 µg/ml), whereas 80 µg/ml fucan 1.5 was needed to obtain the same effect. For both fucans this effect was abolished by desulfation. These polymers are composed of fucose, xylose, uronic acid, galactose, and sulfate at molar ratios of 1.0:0.8:0.7:0.8:0.4 and 1.0:0.3:0.4:1.5:1.3, respectively. This is the fist report indicating the presence of a heterofucan with higher anticoagulant activity from brown seaweed.
Palavra-chave Fucan
Fucoidan
Anticoagulant drugs
Brown algae
Antithrombotic agents
Idioma Inglês
Data de publicação 2004-02-01
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 37, n. 2, p. 167-171, 2004.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Publicador Associação Brasileira de Divulgação Científica
Extensão 167-171
Fonte http://dx.doi.org/10.1590/S0100-879X2004000200002
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000189185500002
SciELO S0100-879X2004000200002 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/1978

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