Identification of genes differentially expressed in hyphae of Candida albicans

Identification of genes differentially expressed in hyphae of Candida albicans

Alternative title Identificação de gases em hifa de Candida albicans
Author Melo, Analy Salles de Azevedo Autor UNIFESP Google Scholar
Serafim, Rui Cosme Autor UNIFESP Google Scholar
Briones, Marcelo Ribeiro da Silva Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The ability to switch from yeast to hyphal forms is essential for Candida albicans virulence. This morphological switch involves the expression of hyphal-specific genes under the control of transcriptional factors. To contribute to the discovery of hyphal-specific genes, we used a differential screening method where clones of a genomic DNA library were hybridized with yeast and hyphal cDNA probes. Two clones with increased expression in hyphae were selected for study. Sequencing these clones, we found that they encoded two important metabolic genes, CaHXT7 (high-affinity hexose transporter) and CaYLL34 (member of the AAA ATPase family). CaHXT7 and CaYLL34 ORFs were completely determined. Analyses of the putative proteins show that: (1) CaHxt7p has one hexose transporter domain and (2) CaYll34p has two AAA ATPase domains. These results show, for the first time, increased expression of metabolic genes in C. albicans hyphae. Also, because the proteins encoded by CaHXT7 and CaYLL34 may be necessary for the switching to hyphae, they could be new targets for antifungal drugs.

A transição morfológica de levedura para hifa é essencial para a virulência de Candida albicans. Esta transição envolve a expressão de genes hifa-específicos que estão sob o controle de fatores transcricionais. Para descobrir genes hifa-específicos utilizamos um método de triagem diferencial, onde clones de biblioteca de DNA genômico foram hibridizados com sondas de cDNA de levedura e hifa. Dois clones com aumento de expressão em hifa foram selecionados. O sequenciamento dos insertos destes clones permitiu a identificação de dois genes metabólicos importantes: CaHXT7 (high-affinity hexose transporter) e CaYLL34 (da família AAA ATPase). As ORFs completas destes genes foram caracterizadas e a análise das proteínas hipotéticas revelou que: (1) CaHxt7p tem um domínio de transportador de hexose e (2) CaYll34 tem dois domínios AAA ATPase. Este é o primeiro estudo que demonstra aumento de expressão de genes metabólicos em hifas de C. albicans. Ainda, a associação dos produtos de CaHXT7 e CaYLL34 com a formação de hifas torna estas proteínas potenciais novos alvos para drogas antifúngicas.
Keywords Differential screening
Candida albicans
HXT7
YLL34
triagem diferencial
Candida albicans
HXT7
YLL34
Language English
Date 2003-11-01
Published in Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 34, p. 135-137, 2003.
ISSN 1517-8382 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira de Microbiologia
Extent 135-137
Origin http://dx.doi.org/10.1590/S1517-83822003000500046
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000207065500046
SciELO ID S1517-83822003000500046 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/1895

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