Parameters affecting cellular invasion and escape from the parasitophorous vacuole by different infective forms of Trypanosoma cruzi

Parameters affecting cellular invasion and escape from the parasitophorous vacuole by different infective forms of Trypanosoma cruzi

Autor Stecconi-Silva, Rb Autor UNIFESP Google Scholar
Andreoli, Wk Autor UNIFESP Google Scholar
Mortara, Renato Arruda Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo In this study we have examined certain aspects of the process of cell invasion and parasitophorous vacuole escape by metacyclic trypomastigotes and extracellular amastigote forms of Trypanosoma cruzi (G strain). Using Vero (and HeLa) cells as targets, we detected differences in the kinetics of vacuole escape by the two forms. Alcalinization of intercellular pH influenced both invasion as well as the escape from the parasitophorous vacuole by metacyclic trypomastigotes, but not the escape kinetics of extracellular amastigotes. We used sialic acid mutants as target cells and observed that the deficiency of this molecule facilitated the escape of both infective forms. Hemolysin activity was only detected in extracellular amastigotes and neither form presented detectable transialidase activity. Invasion of extracellular amastigotes and trypomastigotes in Vero cells was affected in different ways by drugs that interfere with host cell Ca2+ mobilization. These results are in line with previous results that indicate that metacyclic trypomastigotes and extracellular amastigote forms utilize mechanisms with particular features to invade host cells and to escape from their parasitophorous vacuoles.
Palavra-chave Trypanosoma cruz
infective forms
parasitophorous vacuole
escape
invasion
kinetics
Idioma Inglês
Data de publicação 2003-10-01
Publicado em Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 98, n. 7, p. 953-958, 2003.
ISSN 0074-0276 (Sherpa/Romeo)
Publicador Instituto Oswaldo Cruz, Ministério da Saúde
Extensão 953-958
Fonte http://dx.doi.org/10.1590/S0074-02762003000700016
Direito de acesso Acesso aberto Open Access
Tipo Artigo
SciELO S0074-02762003000700016 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/1887

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