Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells

Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells

Author Nunes, Viviane A. Autor UNIFESP Google Scholar
Gozzo, Andrezza Justino Autor UNIFESP Google Scholar
Juliano, Maria Aparecida Autor UNIFESP Google Scholar
Cerqueira César, M. Google Scholar
Sampaio, Misako Uemura Autor UNIFESP Google Scholar
Sampaio, Claudio a.m. Autor UNIFESP Google Scholar
Araujo, Mariana da Silva Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Apoptosis and necrosis are two distinct forms of cell death that can occur in response to different agents and stress conditions. In order to verify if the oxidative stress induced by dietary selenium and vitamin E deficiencies can lead muscle cells to apoptosis, one-day-old chicks were reared using diets differing in their vitamin E (0 or 10 IU/kg) and selenium (0 or 0.15 ppm) supplementation. Chick skeletal muscle tissue was obtained from 28-day-old animals and used to verify apoptosis occurrence based on caspase activity detection and DNA fragmentation. Antioxidant deficiency significantly increased caspase-like activity assessed by the hydrolysis of fluorogenic peptide substrates (Abz-peptidyl-EDDnp) at lambdaexc = 320 nm and lambdaem = 420 nm. Proteolytic activation was not accompanied by typical internucleosomal DNA fragmentation detected by field inversion gel electrophoresis. Although the general caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone (Z-VAD-fmk) (0 to 80 muM) did not block caspase-like activity when preincubated for 30 min with muscle homogenates, the hydrolyzed substrates presented the same cleavage profile in HPLC (at the aspartic acid residue) when incubated with the purified recombinant enzyme caspase-3. These data indicate that oxidative stress causes caspase-like activation in muscle cells and suggest that cell death associated with exudative diathesis (dietary deficiency of selenium and vitamin E) can follow the apoptotic pathway.
Keywords Oxidative stress
Caspase activation
Apoptosis
Dietary selenium deficiency
Dietary vitamin E deficiency
Internucleosomal DNA fragmentation
Caspase substrate
Language English
Date 2003-08-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 8, p. 1047-1053, 2003.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Associação Brasileira de Divulgação Científica
Extent 1047-1053
Origin http://dx.doi.org/10.1590/S0100-879X2003000800010
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000185012900011
SciELO ID S0100-879X2003000800010 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/1809

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