Karyotype of cryopreserved bone marrow cells

Karyotype of cryopreserved bone marrow cells

Autor Chauffaille, Maria de Lourdes Lopes Ferrari Autor UNIFESP Google Scholar
Pinheiro, Ronald Feitosa Autor UNIFESP Google Scholar
Stefano, Jose Tadeu Autor UNIFESP Google Scholar
Kerbauy, José Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo The analysis of chromosomal abnormalities is important for the study of hematological neoplastic disorders since it facilitates classification of the disease. The ability to perform chromosome analysis of cryopreserved malignant marrow or peripheral blast cells is important for retrospective studies. In the present study, we compared the karyotype of fresh bone marrow cells (20 metaphases) to that of cells stored with a simplified cryopreservation method, evaluated the effect of the use of granulocyte-macrophage colony-stimulating factor (GM-CSF) as an in vitro mitotic index stimulator, and compared the cell viability and chromosome morphology of fresh and cryopreserved cells whenever possible (sufficient metaphases for analysis). Twenty-five bone marrow samples from 24 patients with hematological disorders such as acute myeloid leukemia, acute lymphoblastic leukemia, myelodysplastic syndrome, chronic myeloid leukemia, megaloblastic anemia and lymphoma (8, 3, 3, 8, 1, and 1 patients, respectively) were selected at diagnosis, at relapse or during routine follow-up and one sample was obtained from a bone marrow donor after informed consent. Average cell viability before and after freezing was 98.8 and 78.5%, respectively (P < 0.05). Cytogenetic analysis was successful in 76% of fresh cell cultures, as opposed to 52% of cryopreserved samples (P < 0.05). GM-CSF had no proliferative effect before or after freezing. The morphological aspects of the chromosomes in fresh and cryopreserved cells were subjectively the same. The present study shows that cytogenetic analysis of cryopreserved bone marrow cells can be a reliable alternative when fresh cell analysis cannot be done, notwithstanding the reduced viability and lower percent of successful analysis that are associated with freezing.
Palavra-chave Cryopreservation
Karyotype
Bone marrow cells
Idioma Inglês
Data de publicação 2003-07-01
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 7, p. 845-850, 2003.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Publicador Associação Brasileira de Divulgação Científica
Extensão 845-850
Fonte http://dx.doi.org/10.1590/S0100-879X2003000700004
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000184240800004
SciELO S0100-879X2003000700004 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/1775

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