Candida dubliniensis identification in Brazilian yeast stock collection

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dc.contributor.author Mariano, Priscilla de Laet Sant'ana [UNIFESP]
dc.contributor.author Milan, Eveline Pípolo [UNIFESP]
dc.contributor.author Matta, Daniel Archimedes da [UNIFESP]
dc.contributor.author Colombo, Arnaldo Lopes [UNIFESP]
dc.date.accessioned 2015-06-14T13:30:02Z
dc.date.available 2015-06-14T13:30:02Z
dc.date.issued 2003-06-01
dc.identifier http://dx.doi.org/10.1590/S0074-02762003000400020
dc.identifier.citation Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 98, n. 4, p. 533-538, 2003.
dc.identifier.issn 0074-0276
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/1745
dc.description.abstract We investigated the presence of Candida dubliniensis among isolates previously identified as Candida albicans and maintained in a yeast stock collection from 1994 to 2000. All isolates were serotyped and further evaluated for antifungal susceptibility profile. After doing a screening test for C. dubliniensis isolates based on the capability of colonies to grow at 42°C, its final identification was obtained by randomly amplified polymorphic DNA (RAPD) analysis using three different primers. A total of 46 out of 548 screened isolates did not exhibit growth at 42°C and were further genotyped by RAPD. Eleven isolates were identified as C. dubliniensis with RAPD analysis. Regarding serotypes, 81.5% of C. albicans and all C. dubliniensis isolates belonged to serotype A. Of note, 9 out of 11 C. dubliniensis isolates were obtained from patients with acquired immunodeficiency syndrome (Aids) and all of them were susceptible to azoles and amphotericin B. We found 17 (3%) C. albicans isolates that were dose-dependent susceptibility or resistant to azoles. In conclusion, we found a low rate of C. dubliniensis isolates among stock cultures of yeasts previously identified as C. albicans. Most of these isolates were recovered from oral samples of Aids patients and exhibited high susceptibility to amphotericin B and azoles. C. albicans serotype A susceptible to all antifungal drugs is the major phenotype found in our stock culture. en
dc.format.extent 533-538
dc.language.iso eng
dc.publisher Instituto Oswaldo Cruz, Ministério da Saúde
dc.relation.ispartof Memórias do Instituto Oswaldo Cruz
dc.rights Acesso aberto
dc.subject Candida dubliniensis en
dc.subject oral candidiasis en
dc.subject Candida spp. en
dc.subject Aids en
dc.title Candida dubliniensis identification in Brazilian yeast stock collection en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade Federal do Rio Grande do Norte Departamento de Microbiologia e Parasitologia
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP) Laboratório Especial de Micologia
dc.description.affiliation Universidade Federal do Rio Grande do Norte Departamento de Microbiologia e Parasitologia
dc.description.affiliationUnifesp UNIFESP, Laboratório Especial de Micologia
dc.identifier.file S0074-02762003000400020.pdf
dc.identifier.scielo S0074-02762003000400020
dc.identifier.doi 10.1590/S0074-02762003000400020
dc.description.source SciELO
dc.identifier.wos WOS:000183993100020



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