In vitro-induced antibody production in chronic hepatitis C virus infection

In vitro-induced antibody production in chronic hepatitis C virus infection

Autor Oliveira Junior, Evandro Antonio Bentes de Autor UNIFESP Google Scholar
Ferraz, Maria Lucia Cardoso Gomes Autor UNIFESP Google Scholar
Perez, Renata de Mello Autor UNIFESP Google Scholar
Silva, Antonio Eduardo Benedito Autor UNIFESP Google Scholar
Lanzoni, Valeria Pereira Autor UNIFESP Google Scholar
Granato, Celso Francisco Hernandes Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo The objectives of the present study were to assess the in vitro-induced anti-hepatitis C virus (HCV) antibody production (IVIAP) in relation to the clinical, biochemical, virologic and histologic variables of patients with HCV infection. The study included 57 patients (60% males) with HCV infection (anti-HCV and HCV-RNA positive). Alanine aminotransferase (ALT) was elevated in 89% of the patients. Mean viral load was 542,241 copies/ml and histology of the liver showed chronic hepatitis in 27/52 (52%) and cirrhosis in 11/52 (21%) patients. IVIAP levels were determined by immunoenzymatic assay at median absorbance of 0.781 at 450 nm. IVIAP was negative in 14% of the patients. When groups with IVIAP levels above and below the median were compared, high IVIAP levels were associated with the male sex, elevated ALT levels and more advanced disease stage. After logistic regression analysis, advanced histologic damage to the liver remained as the only independent variable associated with elevated IVIAP levels. Using a receiver operator characteristic curve, the best cut-off level for IVIAP was established (= 1.540), with 71% sensitivity and 94% specificity for the detection of more advanced disease stages (grades 3 and 4). These findings are consistent with the participation of immunological mechanisms in the genesis of the hepatic lesions induced by HCV and indicate that the IVIAP test may be useful as a noninvasive marker of liver damage either alone or in combination with other markers.
Palavra-chave Hepatitis C
In vitro-induced antibody production
IVIAP
Hepatitis C virus-RNA
Liver biopsy
Anti-HCV antibodies
Idioma Inglês
Data de publicação 2003-03-01
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 3, p. 361-368, 2003.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Publicador Associação Brasileira de Divulgação Científica
Extensão 361-368
Fonte http://dx.doi.org/10.1590/S0100-879X2003000300011
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000181920300011
SciELO S0100-879X2003000300011 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/1670

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