Protease activity in Giardia duodenalis trophozoites of axenic strains isolated from symptomatic and asymptomatic patients

Protease activity in Giardia duodenalis trophozoites of axenic strains isolated from symptomatic and asymptomatic patients

Autor Guimarães, Semíramis Google Scholar
Sogayar, Maria Inês Terra Leme Google Scholar
Franco, Marcello Fabiano de Autor UNIFESP Google Scholar
Instituição Universidade Estadual Paulista (UNESP)
Universidade Federal de São Paulo (UNIFESP)
Resumo We have examined by gelatin-SDS-PAGE the protease activity in cell lysates of Giardia duodenalis trophozoites of two axenic strains isolated in Brazil from a symptomatic patient (BTU-11) and an asymptomatic carrier (BTU-10), and the reference strain Portland 1 (P1). The proteolysis band patterns showed differences among strains isolated from asymptomatic and symptomatic individuals. The lysate of the strain BTU-10, showed only five hydrolysis bands, while a greater number of bands (10-11 bands) was seen in strains BTU-11 and P1. The protease activity in all lysates was inhibited by cysteine (E-64 and iodoacetamide) and serine proteases (TPCK and TLCK) inhibitors, but not by PMSF and EDTA. In general, the results revealed protease activities in G. duodenalis trophozoites of Brazilian axenic strains and the predominance of cysteine proteinases. It should be stressed the inter-strain difference in hydrolysis band patterns observed between strains isolated from symptomatic patients and the strain obtained from an asymptomatic carrier.
Palavra-chave Giardia duodenalis
axenic strain
trophozoites
protease activity
Idioma Inglês
Data de publicação 2003-01-01
Publicado em Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 98, n. 1, p. 77-81, 2003.
ISSN 0074-0276 (Sherpa/Romeo)
Publicador Instituto Oswaldo Cruz, Ministério da Saúde
Extensão 77-81
Fonte http://dx.doi.org/10.1590/S0074-02762003000100011
Direito de acesso Acesso aberto Open Access
Tipo Artigo
SciELO S0074-02762003000100011 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/1611

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