Avaliação da atividade biológica e estudo da glicosilação da tireotrofina no hipotiroidismo central por necrose hipofisária pós-parto (Síndrome de Sheehan)

Avaliação da atividade biológica e estudo da glicosilação da tireotrofina no hipotiroidismo central por necrose hipofisária pós-parto (Síndrome de Sheehan)

Alternative title Evaluation of the biological activity and study of thyrotropin glycolisation in central hypothyroidism due to post partum pituitary necrosis (Sheehan's syndrome)
Author Oliveira, Juliana Helena Abreu de Autor UNIFESP Google Scholar
Advisor Abucham Filho, Júlio Zaki Autor UNIFESP Google Scholar
Abstract Serum TSH levels are paradoxically elevated in patients with hypothyroidism due to Sheehan's syndrome (Abucham et al 1997 Clin Endocrimol 47:515-22). To further understand this apparent discrepancy, the biological activity and glycosylation pattern of serum TSH was studied in 5 untreated patients with Sheehan's syndrome and in 6 normal controls. TSH bioassay was based on cAMP generation, measured by RIA, in a cell culture system of CHO cells transfected with recombinat human TSH receptor. The oligosaccharide branching of TSH was studied by concanavalin-A (Con-A) lectin affinity chromatography. TSH bioactivity (B/I) was expressed as the ratio between biological and immunofluorimetric assays. Statistical analyses were done by Student's t test or Mann-Whitney U-test. Results are mean ñ SD. Serum FT4 levels were lower in patients than in controls (0.36ñ0.03 µg/dL vs 1.13ñ0.17 µg/dL, respectively, P=0.0001). Circulating immunoreactive TSH was higher in patients with Sheehan's syndrome as compared to controls (4.38ñ1.99 mU/L vs 2.2ñ1.04 mU/L, respectively, P=0.08). In contrast, TSH B/I was significantly decreased in Sheehan's patients as compared to control (0.75ñ0.3 vs 1.9ñ0.8, respectively, P<0.02). The con-A chromatography of serum TSH showed a similar distribution (0.3
Keywords Tireotropina
Hipopituitarismo
Language Portuguese
Date 1998
Published in São Paulo: [s.n.], 1998. 37 p.
Publisher Universidade Federal de São Paulo (UNIFESP)
Extent 37 p.
Access rights Closed access
Type Dissertation
URI http://repositorio.unifesp.br/handle/11600/15642

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