The application of latent class analysis for diagnostic test validation of chronic Trypanosoma cruzi infection in blood donors

The application of latent class analysis for diagnostic test validation of chronic Trypanosoma cruzi infection in blood donors

Autor Langhi Junior, Dante M. Google Scholar
Bordin, Jose Orlando Autor UNIFESP Google Scholar
Castelo Filho, Adauto Autor UNIFESP Google Scholar
Walter, Stephen D. Google Scholar
Moraes-souza, Hélio Google Scholar
Stumpf, Robert J. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo The main strategy to prevent transfusion-associated Chagas' disease is the identification of T. Cruzi-infected blood donors by serological screening tests, however there is no perfect serological gold standard. We evaluated an enzyme immunoassay (EIA), an indirect hemaglutination (IHA), and an indirect immunofluorescence (IIF) test for detecting T. Cruzi antibodies in Brazilian blood donors. The results were submitted to latent class analysis, and a radioimmunopreciptation (RIPA) test was performed on repeatedly positive samples. Among 1951 donors, 11 (0.56%) were positive by EIA, 6 (0.31%) by IHA and 16 (0.82%) by IIF. Six samples were positive with all tests, while 4 reacted with EIA and IIF. The RIPA was positive in 6 (75.0%), 7 (66.6%), and 4 (54.0%) samples reacting by the EIA, IHA and IIF tests, respectively. The latent class model detected a high sensitivity rate (100%) for the EIA and IIF, and a specificity rate of 99.95% and 99.69% for the EIA and IIF tests, respectively. The probability of being case according to the model was 99.92% when both EIA and IIF were positive, and 100% for the association of EIA, IIF, and IHA.
Palavra-chave Chaga's disease
blood donors
diagnostic test
latent class analysis
Idioma Inglês
Data de publicação 2002-08-01
Publicado em Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 6, n. 4, p. 181-187, 2002.
ISSN 1413-8670 (Sherpa/Romeo)
Publicador Brazilian Society of Infectious Diseases
Extensão 181-187
Fonte http://dx.doi.org/10.1590/S1413-86702002000400005
Direito de acesso Acesso aberto Open Access
Tipo Artigo
SciELO S1413-86702002000400005 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/1488

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