TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines

TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines

Autor Dalla Torre, Cristiane Arruda Autor UNIFESP Google Scholar
Maciel, Rui Monteiro de Barros Autor UNIFESP Google Scholar
Pinheiro, Nídia Alice Autor UNIFESP Google Scholar
Andrade, Joyce Anderson Duffles Autor UNIFESP Google Scholar
Toledo, Silvia Regina Caminada de Autor UNIFESP Google Scholar
Villa, Luisa Lina Google Scholar
Cerutti, Janete Maria Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Instituto Ludwig de Pesquisa sobre o Câncer
Resumo Measurement of telomerase activity in clinically obtained tumor samples may provide important information for use as both a diagnostic marker and a prognostic indicator for patient outcome. In order to evaluate telomerase activity in tumor tissue without radiolabeling the product, we developed a simple telomeric repeat amplification protocol-silver-staining assay that is less time-consuming, is safe and requires minimal equipment. In addition, we determined the sensitivity of the silver-staining method by using extracts of telomerase-positive thyroid carcinoma cell lines which were serially diluted from 5,000 to 10 cells. Telomerase activity was also assayed in 19 thyroid tumors, 2 normal controls and 27 bone marrow aspirates. The results indicate that the technique permits the detection of telomerase activity from 5000 to as few as 10 cells. We propose that it could be immediately applicable in many laboratories due to the minimal amount of equipment required.
Palavra-chave Telomeric repeat amplification protocol
TRAP assay
Human thyroid cancer
Leukemia
Telomerase
Silver staining
Idioma Inglês
Data de publicação 2002-01-01
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 35, n. 1, p. 65-68, 2002.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Publicador Associação Brasileira de Divulgação Científica
Extensão 65-68
Fonte http://dx.doi.org/10.1590/S0100-879X2002000100009
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000173942600009
SciELO S0100-879X2002000100009 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/1315

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