Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells

Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells

Author Merjan, A.j. Autor UNIFESP Google Scholar
Kanashiro, Celia Akemi Autor UNIFESP Google Scholar
Krieger, Jose Eduardo Google Scholar
Han, Sang Won Autor UNIFESP Google Scholar
Paiva, Antonio Cechelli de Mattos Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract A construct (AT1R-NF) containing a Flag sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization.
Keywords angiotensin II
agonist-induced receptor internalization
endocytosis
fluorescence-tagged receptor
nuclear angiotensin receptors
renin-angiotensin system
Language English
Date 2001-09-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 9, p. 1175-1183, 2001.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Associação Brasileira de Divulgação Científica
Extent 1175-1183
Origin http://dx.doi.org/10.1590/S0100-879X2001000900011
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000171117600011
SciELO ID S0100-879X2001000900011 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/1236

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