Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells

Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells

Autor Merjan, A.j. Autor UNIFESP Google Scholar
Kanashiro, Celia Akemi Autor UNIFESP Google Scholar
Krieger, Jose Eduardo Google Scholar
Han, Sang Won Autor UNIFESP Google Scholar
Paiva, Antonio Cechelli de Mattos Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Resumo A construct (AT1R-NF) containing a Flag sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization.
Palavra-chave angiotensin II
agonist-induced receptor internalization
endocytosis
fluorescence-tagged receptor
nuclear angiotensin receptors
renin-angiotensin system
Idioma Inglês
Data de publicação 2001-09-01
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 9, p. 1175-1183, 2001.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Publicador Associação Brasileira de Divulgação Científica
Extensão 1175-1183
Fonte http://dx.doi.org/10.1590/S0100-879X2001000900011
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000171117600011
SciELO S0100-879X2001000900011 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/1236

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