Mycobacterium bovis: polymerase chain reaction identification in bovine Lymphonode biopsies and genotyping in isolates from Southeast Brazil by spolygotyping and restriction fragment length polymorphism

Mycobacterium bovis: polymerase chain reaction identification in bovine Lymphonode biopsies and genotyping in isolates from Southeast Brazil by spolygotyping and restriction fragment length polymorphism

Autor Zanini, Ms Google Scholar
Moreira, Ec Google Scholar
Lopes, Mtp Google Scholar
Oliveira, Rs Autor UNIFESP Google Scholar
Leao, Sylvia Cardoso Autor UNIFESP Google Scholar
Fioravanti, Rl Google Scholar
Roxo, E Google Scholar
Zumarraga, M Google Scholar
Romano, Mi Google Scholar
Cataldi, A Google Scholar
Salas, Ce Google Scholar
Instituição ICB Departamento de Bioquímica e Imunologia Laboratório de Biologia Molecular de Produtos Naturais
Universidade Federal de Minas Gerais ICB Escola de Veterinária
Universidade Federal de Minas Gerais ICB Departamento de Farmacologia
Universidade Federal de São Paulo (UNIFESP)
Laboratório Central do Estado do Espírito Santo
Instituto Biológico de São Paulo
Centro de Investigación en Ciencias Veterinarias Instituto de Biotecnologia
Resumo Diagnosis of the Mycobacterium tuberculosis complex by direct PCR of mediastinal lymphnode DNA and microbiological tests were compared in cattle suspicious of bearing tuberculous-like lesions detected during slaughter. The PCR procedure applied on DNA samples (n=54) obtained by adding alpha -casein into the thiocyanate extraction mix was positive in 70% of the samples. PCR confirmed the identification of 23 samples (100%) that grew in culture, 9 samples (60%) that failed to grow in culture, plus 6 (37.5%) samples that resulted in growth of bacterial contaminants. Genotyping by IS6110-RFLP and DR-spoligotyping analysis of seven samples revealed the presence of several polimorphisms. Seven of the isolates contained multiple copies of IS6110, thus defining the existence of five singular genotypes.
Palavra-chave Mycobacterium bovis diagnosis
DNA polimorphism
thiocyanate guanidinium
Brazil
Idioma Inglês
Data de publicação 2001-08-01
Publicado em Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 96, n. 6, p. 809-813, 2001.
ISSN 0074-0276 (Sherpa/Romeo)
Publicador Instituto Oswaldo Cruz, Ministério da Saúde
Extensão 809-813
Fonte http://dx.doi.org/10.1590/S0074-02762001000600012
Direito de acesso Acesso aberto Open Access
Tipo Artigo
SciELO S0074-02762001000600012 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/1226

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