Heparan sulfate and control of cell division: adhesion and proliferation of mutant CHO-745 cells lacking xylosyl transferase

Heparan sulfate and control of cell division: adhesion and proliferation of mutant CHO-745 cells lacking xylosyl transferase

Author Franco, Celia Regina Cavichlolo Autor UNIFESP Google Scholar
Rocha, Hugo Alexandre Oliveira Autor UNIFESP Google Scholar
Trindade, Edvaldo da Silva Autor UNIFESP Google Scholar
Santos, Isabel Anunciacao Neves dos Autor UNIFESP Google Scholar
Leite, Edda Lisboa Autor UNIFESP Google Scholar
Veiga, Silvio Sanches Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Dietrich, Carl Peter Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do Rio Grande do Norte Departamento de Bioquímica
Universidade Federal do Paraná Departamento de Biologia Celular
Abstract We have examined the role of cell surface glycosaminoglycans in cell division: adhesion and proliferation of Chinese hamster ovary (CHO) cells. We used both wild-type (CHO-K1) cells and a mutant (CHO-745) which is deficient in the synthesis of proteoglycans due to lack of activity of xylosyl transferase. Using different amounts of wild-type and mutant cells, little adhesion was observed in the presence of laminin and type I collagen. However, when fibronectin or vitronectin was used as substrate, there was an enhancement in the adhesion of wild-type and mutant cells. Only CHO-K1 cells showed a time-dependent adhesion on type IV collagen. These results suggest that the two cell lines present different adhesive profiles. Several lines of experimental evidence suggest that heparan sulfate proteoglycans play a role in cell adhesion as positive modulators of cell proliferation and as key participants in the process of cell division. Proliferation and cell cycle assays clearly demonstrate that a decrease in the amount of glycosaminoglycans does not inhibit the proliferation of mutant CHO-745 cells when compared to the wild type CHO-K1, in agreement with the findings that both CHO-K1 and CHO-745 cells take 8 h to enter the S phase.
Keywords CHO cells
extracellular matrix proteins
proteoglycans
glycosaminoglycans
cell adhesion
cell proliferation
Language English
Date 2001-08-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 8, p. 971-975, 2001.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Associação Brasileira de Divulgação Científica
Extent 971-975
Origin http://dx.doi.org/10.1590/S0100-879X2001000800001
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000170731800001
SciELO ID S0100-879X2001000800001 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/1215

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