Behavioral and electroencephalographic analysis of seizures induced by intrahippocampal injection of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera

Behavioral and electroencephalographic analysis of seizures induced by intrahippocampal injection of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera

Autor Santana, Alfredo Nicodemus da Cruz Google Scholar
Trindade Filho, Euclides Mauricio Autor UNIFESP Google Scholar
Cunha, R.b. Google Scholar
Sousa, M.v. Google Scholar
Cavalheiro, Esper Abrão Autor UNIFESP Google Scholar
Carvalho, K.m. Google Scholar
Instituição Universidade Estadual do Ceará Departamento de Ciências Fisiológicas Laboratório de Neurofarmacologia
Universidade Federal de São Paulo (UNIFESP)
Universidade de Brasília Departamento de Biologia Celular Centro Brasileiro de Serviços e Pesquisas em Proteínas
Resumo In this study, the behavioral and electroencephalographic (EEG) analysis of seizures induced by the intrahippocampal injection in rats of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera, was determined. The first alterations occurred during microinjection of granulitoxin (8 µg) into the dorsal hippocampus and consisted of seizure activity that began in the hippocampus and spread rapidly to the occipital cortex. This activity lasted 20-30 s, and during this period the rats presented immobility. During the first 40-50 min after its administration, three to four other similar short EEG seizure periods occurred and the rats presented the following behavioral alterations: akinesia, facial automatisms, head tremor, salivation, rearing, jumping, barrel-rolling, wet dog shakes and forelimb clonic movements. Within 40-50 min, the status epilepticus was established and lasted 8-12 h. These results are similar to those observed in the acute phase of the pilocarpine model of temporal lobe epilepsy and suggest that granulitoxin may be a useful tool not only to study the sodium channels, but also to develop a new experimental model of status epilepticus.
Palavra-chave Bunodosoma granulifera
granulitoxin
sea anemones
toxic peptides
seizure
electroencephalographic assay
Idioma Inglês
Data de publicação 2001-06-01
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 6, p. 797-801, 2001.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Publicador Associação Brasileira de Divulgação Científica
Extensão 797-801
Fonte http://dx.doi.org/10.1590/S0100-879X2001000600016
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000169443400016
SciELO S0100-879X2001000600016 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/1188

Exibir registro completo




Arquivo

Nome: S0100-879X2001000600016.pdf
Tamanho: 142.1KB
Formato: PDF
Descrição:
Abrir arquivo

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta