A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins

A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins

Autor Rocha, Hugo Alexandre Oliveira Autor UNIFESP Google Scholar
Franco, Celia Regina Cavichlolo Autor UNIFESP Google Scholar
Trindade, Edvaldo da Silva Autor UNIFESP Google Scholar
Carvalho, L.c.m. Google Scholar
Veiga, Silvio Sanches Autor UNIFESP Google Scholar
Leite, Edda Lisboa Autor UNIFESP Google Scholar
Dietrich, Carl Peter Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Instituição Universidade Federal do Rio Grande do Norte Departamento de Bioquímica
Universidade Federal do Paraná Departamento de Biologia Celular
Universidade Federal de São Paulo (UNIFESP)
Resumo Fucans, a family of sulfated polysaccharides present in brown seaweed, have several biological activities. Their use as drugs would offer the advantage of no potential risk of contamination with viruses or particles such as prions. A fucan prepared from Spatoglossum schröederi was tested as a possible inhibitor of cell-matrix interactions using wild-type Chinese hamster ovary cells (CHO-K1) and the mutant type deficient in xylosyltransferase (CHO-745). The effect of this polymer on adhesion properties with specific extracellular matrix components was studied using several matrix proteins as substrates for cell attachment. Treatment with the polymer inhibited the adhesion of fibronectin to both CHO-K1 (2 x 10(5))()and CHO-745 (2 x 10(5) and 5 x 10(5)) cells. No effect was detected with laminin, using the two cell types. On the other hand, adhesion to vitronectin was inhibited in CHO-K1 cells and adhesion to type I collagen was inhibited in CHO-745 cells. In spite of this inhibition, the fucan did not affect either cell proliferation or cell cycle. These results demonstrate that this polymer is a new anti-adhesive compound with potential pharmacological applications.
Palavra-chave CHO cells
extracellular matrix proteins
fucan
glycosaminoglycans
cell adhesion
brown algae
Idioma Inglês
Data de publicação 2001-05-01
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 5, p. 621-626, 2001.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Publicador Associação Brasileira de Divulgação Científica
Extensão 621-626
Fonte http://dx.doi.org/10.1590/S0100-879X2001000500009
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000168735900009
SciELO S0100-879X2001000500009 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/1159

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