Typing of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis

Mostrar registro simples

dc.contributor.author Bedendo, Joao [UNIFESP]
dc.contributor.author Pignatari, Antonio Carlos Campos [UNIFESP]
dc.date.accessioned 2015-06-14T13:25:07Z
dc.date.available 2015-06-14T13:25:07Z
dc.date.issued 2000-11-01
dc.identifier http://dx.doi.org/10.1590/S0100-879X2000001100002
dc.identifier.citation Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 33, n. 11, p. 1269-1274, 2000.
dc.identifier.issn 0100-879X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/1047
dc.description.abstract Polymerase chain reaction (PCR) with JB1 or REP consensus oligonucleotides and pulsed field gel electrophoresis (PFGE) were used to study genomic DNA extracted from 31 strains of enterococci. Eleven ATCC strains, representative of 11 species of Enterococcus, were initially tested by JB1-PCR, revealing that Enterococcus malodoratus and Enterococcus hirae presented identical banding patterns. Eight Enterococcus faecium isolates from Stanford University and 12 from São Paulo Hospital were studied by JB1-PCR, REP-PCR 1/2R and PFGE. Among the isolates from Stanford University, 5 genotypes were defined by JB1-PCR, 7 by REP-PCR 1/2R and 4 by PFGE. Among the isolates from São Paulo Hospital, 9 genotypes were identified by JB1-PCR, 6 by REP-PCR and 5 by PFGE. The three methods identified identical genotypes, but there was not complete agreement among them. en
dc.format.extent 1269-1274
dc.language.iso eng
dc.publisher Associação Brasileira de Divulgação Científica
dc.relation.ispartof Brazilian Journal of Medical and Biological Research
dc.rights Acesso aberto
dc.subject molecular typing en
dc.subject Enterococcus faecium en
dc.subject PCR-PFGE en
dc.title Typing of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis en
dc.type Artigo
dc.contributor.institution Universidade Estadual de Maringá Centro de Ciências da Saúde Departamento de Enfermagem
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Estadual de Maringá Centro de Ciências da Saúde Departamento de Enfermagem
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP) Departamento de Doenças Infecciosas e Parasitárias
dc.description.affiliationUnifesp UNIFESP, Depto. de Doenças Infecciosas e Parasitárias
dc.identifier.file S0100-879X2000001100002.pdf
dc.identifier.scielo S0100-879X2000001100002
dc.identifier.doi 10.1590/S0100-879X2000001100002
dc.description.source SciELO
dc.identifier.wos WOS:000165487100002



Arquivos deste item

Nome: S0100-879X2000001100002.pdf
Tamanho: 316.9Kb
Formato: PDF
Descrição:
Visualizar/Abrir

Este item aparece na(s) seguinte(s) coleção(s)

Mostrar registro simples