Typing of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis

Typing of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis

Autor Bedendo, Joao Autor UNIFESP Google Scholar
Pignatari, Antonio Carlos Campos Autor UNIFESP Google Scholar
Instituição Universidade Estadual de Maringá Centro de Ciências da Saúde Departamento de Enfermagem
Universidade Federal de São Paulo (UNIFESP)
Resumo Polymerase chain reaction (PCR) with JB1 or REP consensus oligonucleotides and pulsed field gel electrophoresis (PFGE) were used to study genomic DNA extracted from 31 strains of enterococci. Eleven ATCC strains, representative of 11 species of Enterococcus, were initially tested by JB1-PCR, revealing that Enterococcus malodoratus and Enterococcus hirae presented identical banding patterns. Eight Enterococcus faecium isolates from Stanford University and 12 from São Paulo Hospital were studied by JB1-PCR, REP-PCR 1/2R and PFGE. Among the isolates from Stanford University, 5 genotypes were defined by JB1-PCR, 7 by REP-PCR 1/2R and 4 by PFGE. Among the isolates from São Paulo Hospital, 9 genotypes were identified by JB1-PCR, 6 by REP-PCR and 5 by PFGE. The three methods identified identical genotypes, but there was not complete agreement among them.
Palavra-chave molecular typing
Enterococcus faecium
PCR-PFGE
Idioma Inglês
Data de publicação 2000-11-01
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 33, n. 11, p. 1269-1274, 2000.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Publicador Associação Brasileira de Divulgação Científica
Extensão 1269-1274
Fonte http://dx.doi.org/10.1590/S0100-879X2000001100002
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000165487100002
SciELO S0100-879X2000001100002 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/1047

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