Typing of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis

Typing of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis

Author Bedendo, Joao Autor UNIFESP Google Scholar
Pignatari, Antonio Carlos Campos Autor UNIFESP Google Scholar
Institution Universidade Estadual de Maringá Centro de Ciências da Saúde Departamento de Enfermagem
Universidade Federal de São Paulo (UNIFESP)
Abstract Polymerase chain reaction (PCR) with JB1 or REP consensus oligonucleotides and pulsed field gel electrophoresis (PFGE) were used to study genomic DNA extracted from 31 strains of enterococci. Eleven ATCC strains, representative of 11 species of Enterococcus, were initially tested by JB1-PCR, revealing that Enterococcus malodoratus and Enterococcus hirae presented identical banding patterns. Eight Enterococcus faecium isolates from Stanford University and 12 from São Paulo Hospital were studied by JB1-PCR, REP-PCR 1/2R and PFGE. Among the isolates from Stanford University, 5 genotypes were defined by JB1-PCR, 7 by REP-PCR 1/2R and 4 by PFGE. Among the isolates from São Paulo Hospital, 9 genotypes were identified by JB1-PCR, 6 by REP-PCR and 5 by PFGE. The three methods identified identical genotypes, but there was not complete agreement among them.
Keywords molecular typing
Enterococcus faecium
PCR-PFGE
Language English
Date 2000-11-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 33, n. 11, p. 1269-1274, 2000.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Associação Brasileira de Divulgação Científica
Extent 1269-1274
Origin http://dx.doi.org/10.1590/S0100-879X2000001100002
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000165487100002
SciELO ID S0100-879X2000001100002 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/1047

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