Evaluation of an enzyme immunoassay for hepatitis C virus antibody detection using a recombinant protein derived from the core region of hepatitis C virus genome

Evaluation of an enzyme immunoassay for hepatitis C virus antibody detection using a recombinant protein derived from the core region of hepatitis C virus genome

Autor Lopes, Epa Autor UNIFESP Google Scholar
Granato, Celso Francisco Hernandes Autor UNIFESP Google Scholar
Lanzoni, Valeria Pereira Autor UNIFESP Google Scholar
Granero, L Autor UNIFESP Google Scholar
Paranhos-Baccala, G Google Scholar
Tomiyama, H Autor UNIFESP Google Scholar
Silva, Aeb Autor UNIFESP Google Scholar
Ferraz, Maria Lucia Cardoso Gomes Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Instituto BioMérieux
Resumo This study was undertaken to evaluate an enzyme immunoassay (EIA) for hepatitis C virus antibody detection (anti-HCV), using just one antigen. Anti-HCV EIA was designed to detect anti-HCV IgG using on the solid-phase a recombinant C22 antigen localized at the N-terminal end of the core region of HCV genome, produced by BioMérieux. The serum samples diluted in phosphate buffer saline were added to wells coated with the C22, and incubated. After washings, the wells were loaded with conjugated anti-IgG, and read in a microtiter plate reader (492 nm). Serum samples of 145 patients were divided in two groups: a control group of 39 patients with non-C hepatitis (10 acute hepatitis A, 10 acute hepatitis B, 9 chronic hepatitis B, and 10 autoimmune hepatitis) and a study group consisting of 106 patients with chronic HCV hepatitis. In the study group all patients had anti-HCV detected by a commercially available EIA (Abbott®), specific for HCV structural and nonstructural polypeptides, alanine aminotransferase elevation or positive serum HCV-RNA detected by nested-PCR. They also had a liver biopsy compatible with chronic hepatitis. The test was positive in 101 of the 106 (95%) sera from patients in the study group and negative in 38 of the 39 (97%) sera from those in the control group, showing an accuracy of 96%. According to these results, our EIA could be used to detect anti-HCV in the serum of patients infected with hepatitis C virus.
Palavra-chave hepatitis C virus (HCV)
anti-HCV antibody
enzyme immunoassay
Idioma Inglês
Data de publicação 2000-10-01
Publicado em Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 95, n. 5, p. 717-720, 2000.
ISSN 0074-0276 (Sherpa/Romeo)
Publicador Instituto Oswaldo Cruz, Ministério da Saúde
Extensão 717-720
Fonte http://dx.doi.org/10.1590/S0074-02762000000500019
Direito de acesso Acesso aberto Open Access
Tipo Artigo
SciELO S0074-02762000000500019 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/1041

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